巨噬示踪

Fluorescent DiI Liposomes

Fluorescent DiI Liposomes: 荧光染料DiI标记的脂质体。 Fluorescent DiI Liposomes利用单核/巨噬细胞的吞噬属性对其荧光标记，可流式细胞术（FACS）分析，共聚焦荧光显微镜（Confocal Microscopy ）检测和体内的生物分布（Biodistribution）来示踪。

DiI是一种亲脂性长链碳菁染料，同系列还包含DiA，DiB，DiD，DiO 和 DiR。碳菁染料广泛用作亲脂性示踪剂，用于标记细胞、细胞器、脂质体、病毒和脂蛋白。DiI在进入细胞膜之前荧光非常弱，仅当进入到细胞膜后才可以被激发出很强的荧光。

Fluorescent DiI Liposomes通过与PBS Liposomes（Blank ），Clodronate Liposomes相同的机制被单核细胞/巨噬细胞识别和吞噬。和PBS Liposomes（Blank ）对照脂质体一样，不含氯膦酸盐，因此单核细胞/巨噬细胞不会被清除。然而，单核细胞/巨噬细胞确实/可能通过细胞因子分泌、吞噬活性的暂时中止和文献中描述的其他反应来对脂质体的摄入产生反应。

Highlights:

* Real-time tracking of movement, accumulation and biodistribution

* Image applications such as FACS, fluorescent microscopy and bioimaging

货号 品名 规格 库存 价格
CLD-8911-2ml
 Fluorescent DiI Liposomes 2ml in stock 2200.0
CLD-8911-5ml
 Fluorescent DiI Liposomes 5ml in stock 4300.0
CLD-8911-10ml
 Fluorescent DiI Liposomes 10ml in stock 7980.0
CLD-8911-15ml
 Fluorescent DiI Liposomes 15ml in stock 10900.0
CLD-8911-20ml
 Fluorescent DiI Liposomes 20ml in stock 14500.0
CLD-8911-25ml
 Fluorescent DiI Liposomes 25ml in stock 17500.0
产品详情

品类型/Product Type:Chemicals
产品名称/Name:Fluorescent DiI Liposomes
脂质构成/Lipid Composition:Cholesterol, L-alpha-Phosphatidylcholine
包裹药物/Encapsulated Drug:DiI (1,1'-Dioctadecyl-3,3,3',3'- Tetramethylindocarbocyanine Perchlorate)
外观形态/Format:Pink liquid suspensions
激发波长/Excitation:549 nm
发射波长/Emission:565nm
缓冲液/Buffer: Phosphate buffered saline (20 mM sodium phosphate, 0.9 % NaCl)
PH值/PH: 7.0-7.5
保存期/Shelf Life：3 months guaranteed for unopened vials at 2-8 ºC
注意事项/Comments: Liposomes should be stored at in the dark at 4 °C and NEVER be frozen
存储温度/Storage: Upon receipt store at 2-8℃
运输温度/Shipped:Ice pack  

文档资料

PDF-logo Technical-Information-for-Fluorescent Liposomes DiI

产品源头

Country of origin: USA

参考文献

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  64. Karupiah G, Buller RM, Van Rooijen N, Duarte CJ, Chen J. Different roles for CD4+ and CD8+ T lymphocytes and macrophage subsets in the control of a generalized virus infection. J Virol. 1996 Dec;70(12):8301-9. doi: 10.1128/JVI.70.12.8301-8309.1996. PMID: 8970949; PMCID: PMC190917. View article

  65. de Haan A, Groen G, Prop J, van Rooijen N, Wilschut J. Mucosal immunoadjuvant activity of liposomes: role of alveolar macrophages. Immunology. 1996 Dec;89(4):488-93. doi: 10.1046/j.1365-2567.1996.d01-787.x. PMID: 9014811; PMCID: PMC1456575. View article

  66. Wolff G, Worgall S, van Rooijen N, Song WR, Harvey BG, Crystal RG. Enhancement of in vivo adenovirus-mediated gene transfer and expression by prior depletion of tissue macrophages in the target organ. J Virol. 1997 Jan;71(1):624-9. doi: 10.1128/JVI.71.1.624-629.1997. PMID: 8985392; PMCID: PMC191093.. View article

  67. van Rooijen N, Sanders A. Elimination, blocking, and activation of macrophages: three of a kind? J Leukoc Biol. 1997 Dec;62(6):702-9. doi: 10.1002/jlb.62.6.702. PMID: 9400810. View article